Role of finasteride in the treatment of induced benign prostatic hyperplasia with accompanying histopathological and DNA damage changes in Albino Mice

Shukur Mahmood Yaseen (1) , Saad Ahmed Ali Jadoo (2)
(1) Medical Biology and Anatomy Department, College of Medicine, University of Diyala, 32001, Diyala, Iraq , Iraq
(2) Department of Family and Community Medicine, University of Diyala, 32001, Diyala, Iraq , Iraq

Abstract

Background: Finasteride was used to treat benign prostatic hyperplasia. It is a typical benign prostatic hyperplasia (BPH) medication, reduces prostate volume and symptoms. This study aimed to evaluate the effectiveness of finasteride in treating BPH by assessing histopathological changes, serum testosterone, PSA levels, and prostate DNA damage. 


Methods: Thirty-five mature male albino mice (aged 7-8 weeks, weighing 25–27 gm) were divided into two groups: control (n=10) and positive treatment (n=25). BPH was induced in the treatment group using testosterone propionate (20 mg/kg) subcutaneously for 30 days. Five mice were sacrificed to confirm effective disease induction. The remaining 20 mice were randomly divided into two subgroups: one (n=10) received distilled water orally as a positive control, while the other (n=10) was treated with finasteride (0.878 mg/kg) daily for 30 days. At the experiment's conclusion, blood samples were collected via cardiac puncture to measure serum testosterone and PSA levels. Mice were then sacrificed under anesthesia, and prostate lobes were isolated for histopathological analysis and DNA damage assessment in each group.


Results: BPH induction resulted in significant prostate hyperplasia, abnormal proliferation of epithelial and stromal cells, and increased inflammation in the prostate tissue of the positive group, leading to acini stenosis. The finasteride-treated group exhibited a mild reduction in hyperplasia, epithelial layer thickness, and inflammation. Serum analysis revealed significantly elevated PSA and testosterone levels in the positive group, which were notably reduced in the finasteride-treated group (P<0.05). Additionally, there was a significant increase in prostate DNA damage in the positive group compared to the control group, with a slight decrease observed in the finasteride-treated group.


Conclusion: These findings demonstrate finasteride’s efficacy in reducing cell proliferation, inflammation, and DNA damage in BPH-induced mice.

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Authors

Shukur Mahmood Yaseen
shuker.m2015@gmail.com (Primary Contact)
Saad Ahmed Ali Jadoo
Role of finasteride in the treatment of induced benign prostatic hyperplasia with accompanying histopathological and DNA damage changes in Albino Mice. (2024). Journal of Lifelong DentoMedical Health, 1(1), 27-32. https://jldmhealth.com/Jldmh/article/view/9

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Role of finasteride in the treatment of induced benign prostatic hyperplasia with accompanying histopathological and DNA damage changes in Albino Mice. (2024). Journal of Lifelong DentoMedical Health, 1(1), 27-32. https://jldmhealth.com/Jldmh/article/view/9
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